7OA7

PilC minor pilin of Streptococcus sanguinis 2908 type IV pili

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-14
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	96.481, 96.481, 85.078
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	83.560 - 1.450
Rwork	0.183
R-free	0.20100
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.000
Data reduction software	xia2
Data scaling software	xia2
Phasing software	autoSHARP
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.080	1.470
High resolution limit [Å]	1.450	1.450
Number of reflections	81269	4043
<I/σ(I)>	15.4
Completeness [%]	100.0
Redundancy	19.97
CC(1/2)	0.999	0.541

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium sulphate; 0.01 M cadmium chloride hemi (pentahydrate); 0.1 M Pipes (pH 7.0); 15 % v/v PEG smear broad; 10 % v/v ethylene glycol