7O9X
Crystal structure of Human Menin with fragment 16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 107.063, 107.063, 107.243 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.944 - 2.299 |
R-factor | 0.2006 |
Rwork | 0.198 |
R-free | 0.24930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ddc |
RMSD bond length | 0.007 |
RMSD bond angle | 0.861 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.381 |
High resolution limit [Å] | 2.299 | 2.299 |
Rmerge | 0.123 | |
Rmeas | 0.130 | |
Rpim | 0.040 | |
Number of reflections | 28344 | 2761 |
<I/σ(I)> | 12.4 | |
Completeness [%] | 99.9 | 99.42 |
Redundancy | 10.9 | |
CC(1/2) | 0.994 | 0.953 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 12% PEG 3350 14% Glycerol |