7O9V
hypothetical protein OMM_04225 residues 244-274 from Candidatus Magnetoglobus multicellularis fused to GCN4 adaptors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.827, 34.649, 82.547 |
| Unit cell angles | 90.00, 93.48, 90.00 |
Refinement procedure
| Resolution | 37.040 - 1.990 |
| R-factor | 0.2656 |
| Rwork | 0.262 |
| R-free | 0.31490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yny |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.733 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.040 | 2.120 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.124 | 1.671 |
| Number of reflections | 11390 | 570 |
| <I/σ(I)> | 8.5 | 1.1 |
| Completeness [%] | 89.3 | 81.4 |
| Redundancy | 6.5 | 6.4 |
| CC(1/2) | 0.998 | 0.657 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2M KSCN, 0.1M bis tris propane pH 8.5, 20% (w/v) PEG 3350 |
