7O9O

Crystal structure of the Awp3b (adhesin-like wall protein 3b) A-domain from Candida glabrata

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	H 3 2
Unit cell lengths	147.970, 147.970, 117.770
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.280 - 1.550
R-factor	0.1608
Rwork	0.159
R-free	0.18820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Awp3bA-Gd
RMSD bond length	0.008
RMSD bond angle	0.992
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.280	1.610
High resolution limit [Å]	1.550	1.550
Number of reflections	69278	6889
<I/σ(I)>	10.68	1.84
Completeness [%]	97.0	97.3
Redundancy	1.9	1.9
CC(1/2)	0.999	0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1 M sodium phosphate, 0.1 M potassium phosphate, 0.1 M MES pH6.5, 2.0 M sodium chloride