7O9O
Crystal structure of the Awp3b (adhesin-like wall protein 3b) A-domain from Candida glabrata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 147.970, 147.970, 117.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.280 - 1.550 |
| R-factor | 0.1608 |
| Rwork | 0.159 |
| R-free | 0.18820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Awp3bA-Gd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.992 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.280 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 69278 | 6889 |
| <I/σ(I)> | 10.68 | 1.84 |
| Completeness [%] | 97.0 | 97.3 |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.999 | 0.431 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M sodium phosphate, 0.1 M potassium phosphate, 0.1 M MES pH6.5, 2.0 M sodium chloride |
