7O9H

Escherichia coli FtsY in complex with pppGpp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-13
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.976253
Spacegroup name	P 21 21 21
Unit cell lengths	74.480, 90.710, 106.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.350 - 2.400
R-factor	0.2061
Rwork	0.203
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yhs
RMSD bond length	0.010
RMSD bond angle	1.220
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.350	2.486
High resolution limit [Å]	2.400	2.400
Number of reflections	161630	18544
<I/σ(I)>	8.85
Completeness [%]	90.8
Redundancy	6.2
CC(1/2)	0.993	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	8.5% isopropanol, 0.085 M HEPES pH 7.5, 17% PEG 4000, 15 % glycerol