7O9G

Escherichia coli Ffh in complex with ppGpp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97626
Spacegroup name	P 1 21 1
Unit cell lengths	48.630, 38.200, 78.440
Unit cell angles	90.00, 96.27, 90.00

Refinement procedure

Resolution	43.260 - 2.800
R-factor	0.1952
Rwork	0.192
R-free	0.25980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ng1
RMSD bond length	0.011
RMSD bond angle	1.635
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.260	2.904
High resolution limit [Å]	2.800	2.803
Number of reflections	7083	698
<I/σ(I)>	32.05
Completeness [%]	97.4
Redundancy	3.4
CC(1/2)	0.999	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium acetate, 20 % (w/v) PEG 3350