7O97
hypothetical protein UY81_C0065G0003 from Candidatus Giovannonibacteria bacterium converted into a canonical coiled coil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 3 |
| Unit cell lengths | 37.203, 37.203, 120.707 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.220 - 2.000 |
| R-factor | 0.2362 |
| Rwork | 0.234 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wpq |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.098 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.220 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.086 | 0.933 |
| Number of reflections | 12693 | 1967 |
| <I/σ(I)> | 15.53 | 1.91 |
| Completeness [%] | 99.5 | 96.8 |
| Redundancy | 9.64 | 9.13 |
| CC(1/2) | 1.000 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2M K2SO4, 20% (w/v) PEG 3350 |
