7O92
hypothetical protein UY81_C0065G0003 residues 18-54 from Candidatus Giovannonibacteria bacterium fused to GCN4 adaptors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.089, 39.842, 78.210 |
| Unit cell angles | 90.00, 103.36, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.700 |
| R-factor | 0.2726 |
| Rwork | 0.269 |
| R-free | 0.31390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yny |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.922 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.860 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.051 | 0.610 |
| Number of reflections | 7604 | 1185 |
| <I/σ(I)> | 13.12 | 1.67 |
| Completeness [%] | 99.1 | 96.7 |
| Redundancy | 3.28 | 3.15 |
| CC(1/2) | 1.000 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1M sodium acetate (pH 4.6), 2M sodium formate |
