7O8S

NmHR light state structure at 32.5 ms (30 - 35 ms) after photoexcitation determined by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	298
Detector technology	PIXEL
Collection date	2019-05-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	104.480, 51.180, 78.330
Unit cell angles	90.00, 131.78, 90.00

Refinement procedure

Resolution	38.960 - 2.500
R-factor	0.2945
Rwork	0.293
R-free	0.32160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b2n
Data reduction software	CrystFEL (v0.8.0)
Data scaling software	CrystFEL (v0.8.0)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	2.040
High resolution limit [Å]	2.000	2.000
Number of reflections	21052	2105
<I/σ(I)>	8.6
Completeness [%]	100.0
Redundancy	373
CC(1/2)	0.990	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	4.5	293	50 mM sodium acetate (pH 4.5), 150 mM MgCl2, 100 mM NaCl, 34% PEG400