7O8L
NmHR dark state structure determined by serial millisecond crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2019-05-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.480, 51.180, 78.330 |
| Unit cell angles | 90.00, 131.78, 90.00 |
Refinement procedure
| Resolution | 38.960 - 1.800 |
| R-factor | 0.1664 |
| Rwork | 0.165 |
| R-free | 0.18560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b2n |
| Data reduction software | CrystFEL (v0.8.0) |
| Data scaling software | CrystFEL (v0.8.0) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 28806 | 2897 |
| <I/σ(I)> | 30.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6719 | |
| CC(1/2) | 1.000 | 0.610 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 4.5 | 293 | 50 mM sodium acetate (pH 4.5), 150 mM MgCl2, 100 mM NaCl, 34% PEG400 |
