7O8C

Structure of SGBP BO2743 from Bacteroides ovatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-13
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.968
Spacegroup name	I 4 2 2
Unit cell lengths	207.120, 207.120, 170.584
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.560 - 2.000
R-factor	0.202
Rwork	0.201
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j5u
RMSD bond length	0.008
RMSD bond angle	0.880
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PDB-REDO (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.560	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	123741	9068
<I/σ(I)>	13.1
Completeness [%]	100.0
Redundancy	20
CC(1/2)	0.845	0.338

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein at 13.7 mg/mL; 2.0 M Ammonium Sulphate, 0.1 M BIS-Tris pH 5.5