7O88
Cystal structure of Zymogen Granule Protein 16 (ZG16)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.978, 45.418, 146.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.388 - 1.200 |
| R-factor | 0.1736 |
| Rwork | 0.173 |
| R-free | 0.19140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3apa |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.388 | 1.243 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.082 | |
| Rpim | 0.045 | |
| Number of reflections | 92432 | 8866 |
| <I/σ(I)> | 8.01 | |
| Completeness [%] | 97.3 | 94.98 |
| Redundancy | 3.7 | 3.5 |
| CC(1/2) | 0.998 | 0.503 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M sodium thiocyanate, 20 % w/v polyethylene glycol (PEG) 3350 |
