7O84
Structure of the PL6 family alginate lyase Pedsa0632 from Pseudopedobacter saltans in complex with substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.07227 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.182, 47.583, 116.200 |
Unit cell angles | 90.00, 98.25, 90.00 |
Refinement procedure
Resolution | 43.970 - 2.177 |
R-factor | 0.2312 |
Rwork | 0.228 |
R-free | 0.28620 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | native Pedsa0632 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | BUSTER (2.10.3 (29-NOV-2019)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.580 | 2.310 |
High resolution limit [Å] | 2.170 | 2.170 |
Number of reflections | 46036 | 7197 |
<I/σ(I)> | 4.8 | |
Completeness [%] | 97.8 | |
Redundancy | 3.3 | |
CC(1/2) | 0.990 | 0.430 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M Amm chloride, 20% (w/v) PEG 3350 |