7O7Y

Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution)

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	GIF Quantum LS
Detector	GATAN K3 (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.2
Software	RELION,cryoSPARC,EPU,Gctf
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION