7O7W
Crystal structure of rsEGFP2 mutant V151L in the non-fluorescent off-state the determined by serial femtosecond crystallography at room temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SACLA BEAMLINE BL2 |
Synchrotron site | SACLA |
Beamline | BL2 |
Temperature [K] | 277.15 |
Detector technology | CCD |
Collection date | 2018-07-28 |
Detector | MPCCD |
Wavelength(s) | 1.631 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.850, 62.930, 72.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.280 - 2.100 |
R-factor | 0.1754 |
Rwork | 0.173 |
R-free | 0.21290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6t39 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.566 |
Data reduction software | CrystFEL (0.7.0) |
Data scaling software | CrystFEL (0.8.0) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.280 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 14734 | 958 |
<I/σ(I)> | 4.9 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 53 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 8 | 293 | 100 mM HEPES pH 8.0, 2 M ammonium sulphate |