7O7T
Structure of the PL6 family alginate lyase Patl3640 from Pseudoalteromonas atlantica T6c in complex with 4-deoxy-L-erythro-5-hexoseulose uronic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 78.232, 78.232, 266.799 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.530 - 2.050 |
R-factor | 0.2108 |
Rwork | 0.209 |
R-free | 0.24310 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | Patl3640 (PDB ID 7O77) |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.530 | 2.170 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 60589 | 9328 |
<I/σ(I)> | 18 | |
Completeness [%] | 99.9 | |
Redundancy | 13 | |
CC(1/2) | 0.990 | 0.770 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 292 | 0.1 M Mg acetate, 0.1 M Na nitrate, 8% (w/v) PEG 10000, 0.15 mM CYMAL-7 |