7O7E
Crystal structure of rsEGFP2 mutant V151L in the fluorescent on-state determined by synchrotron radiation at 100K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.144, 62.512, 70.418 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.380 - 1.800 |
| R-factor | 0.1616 |
| Rwork | 0.159 |
| R-free | 0.20760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dtx |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.380 | 1.880 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.065 | 0.466 |
| Number of reflections | 21205 | 2482 |
| <I/σ(I)> | 14.17 | 2.72 |
| Completeness [%] | 98.3 | 98.3 |
| Redundancy | 4.3 | 4.3 |
| CC(1/2) | 0.999 | 0.916 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 1.7 M ammonium sulfate, 100 mM HEPES pH 7.8 |
