7O6W
Crystal structure of (the) VEL1 VEL polymerising domain (I664D mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979500 |
Spacegroup name | P 63 |
Unit cell lengths | 79.259, 79.259, 59.957 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.490 - 2.640 |
R-factor | 0.21527 |
Rwork | 0.212 |
R-free | 0.24916 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.012 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.490 | 10.000 | 2.770 |
High resolution limit [Å] | 2.640 | 8.000 | 2.640 |
Rmerge | 0.068 | 0.033 | 1.045 |
Rmeas | 0.069 | 0.033 | 1.000 |
Rpim | 0.011 | 0.050 | 0.183 |
Number of reflections | 6333 | 186 | 782 |
<I/σ(I)> | 37 | ||
Completeness [%] | 98.8 | 97.2 | 92 |
Redundancy | 20 | 15 | 15 |
CC(1/2) | 1.000 | 1.000 | 0.842 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.25M (NH4)H2PO4 |