7O6U
Crystal structure of the VIN3 VEL polymerising domain (R554A R556D mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-18 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97950 |
Spacegroup name | I 41 |
Unit cell lengths | 40.758, 40.758, 83.966 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.840 - 1.840 |
R-factor | 0.22364 |
Rwork | 0.221 |
R-free | 0.24438 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.507 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.840 | 1.880 |
High resolution limit [Å] | 1.840 | 1.840 |
Number of reflections | 5941 | 354 |
<I/σ(I)> | 39.2 | |
Completeness [%] | 99.7 | |
Redundancy | 13.8 | |
CC(1/2) | 1.000 | 0.906 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 15% v/v ethanol, 0.1M citrate pH 5.5, 0.2M lithium sulfate |