7O6U

Crystal structure of the VIN3 VEL polymerising domain (R554A R556D mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97950
Spacegroup name	I 41
Unit cell lengths	40.758, 40.758, 83.966
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.840 - 1.840
R-factor	0.22364
Rwork	0.221
R-free	0.24438
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.507
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.840	1.880
High resolution limit [Å]	1.840	1.840
Number of reflections	5941	354
<I/σ(I)>	39.2
Completeness [%]	99.7
Redundancy	13.8
CC(1/2)	1.000	0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	15% v/v ethanol, 0.1M citrate pH 5.5, 0.2M lithium sulfate