7O6T

Crystal structure of the polymerising VEL domain of VIN3 (R556D I575D mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	31.423, 51.809, 85.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.490 - 2.020
Rwork	0.202
R-free	0.23655
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.228
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.490	2.070
High resolution limit [Å]	2.020	2.020
Number of reflections	9677	677
<I/σ(I)>	36.2
Completeness [%]	99.7
Redundancy	20
CC(1/2)	1.000	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.06M Morpheus Divalents, 0.1M Morpheus Buffer System 2 pH 7.5, 10% w/v PEG 8K, 20% v/v ethylene glycol