7O6C
Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 15 minutes under anaerobic environment
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.87000 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 182.449, 182.449, 182.449 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.720 - 1.200 |
| R-factor | 0.1222 |
| Rwork | 0.121 |
| R-free | 0.14250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r03 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.469 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 55.010 | 2.320 | 1.260 |
| High resolution limit [Å] | 1.200 | 2.200 | 1.200 |
| Rmerge | 0.066 | 0.115 | 0.760 |
| Rmeas | 0.070 | 0.139 | 0.817 |
| Rpim | 0.015 | 0.056 | 0.215 |
| Number of reflections | 81103 | 1749 | 11665 |
| <I/σ(I)> | 22 | 7.1 | 3.3 |
| Completeness [%] | 100.0 | 91.1 | 100 |
| Redundancy | 20.1 | 5.5 | 14.1 |
| CC(1/2) | 1.000 | 0.984 | 0.869 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | 1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0 | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | 1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0 | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | 1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0 |
