7O6A
Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 5 minutes under anaerobic environment
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-17 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.90001 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 183.675, 183.675, 183.675 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 106.040 - 1.400 |
R-factor | 0.1331 |
Rwork | 0.132 |
R-free | 0.16250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r03 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.412 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 106.040 | 2.210 | 1.480 |
High resolution limit [Å] | 1.400 | 2.100 | 1.400 |
Rmerge | 0.067 | 0.149 | 0.904 |
Rmeas | 0.070 | 0.193 | 0.944 |
Rpim | 0.015 | 0.104 | 0.200 |
Number of reflections | 52581 | 1869 | 7551 |
<I/σ(I)> | 26.3 | 4.9 | 3.7 |
Completeness [%] | 100.0 | 83.7 | 100 |
Redundancy | 22 | 2.8 | 22.2 |
CC(1/2) | 1.000 | 0.953 | 0.899 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | 1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0 | |
1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | 1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0 | |
1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | 1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0 |