7O64

Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 1 minute.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97622
Spacegroup name	F 4 3 2
Unit cell lengths	184.168, 184.168, 184.168
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.590 - 1.960
R-factor	0.1647
Rwork	0.163
R-free	0.20430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r03
RMSD bond length	0.009
RMSD bond angle	1.692
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.110	3.000	2.070
High resolution limit [Å]	1.960	2.850	1.960
Rmerge	0.166	0.611	0.672
Rmeas	0.172	0.635	0.696
Rpim	0.037	0.140	0.149
Number of reflections	17419	936	2829
<I/σ(I)>	11.3	2.9	3.4
Completeness [%]	87.7	100	100
Redundancy	21	20.1	21.5
CC(1/2)	0.997	0.950	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		281.15	1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0
1	VAPOR DIFFUSION, HANGING DROP		281.15	1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0
1	VAPOR DIFFUSION, HANGING DROP		281.15	1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0