7O60
Crystal structure of human myelin protein P2 at room temperature from joint X-ray and neutron refinement.
Experimental procedure
Experimental method | LAUE |
Source type | NUCLEAR REACTOR |
Source details | ILL BEAMLINE LADI III |
Synchrotron site | ILL |
Beamline | LADI III |
Temperature [K] | 292 |
Detector technology | AREA DETECTOR |
Collection date | 2014-09-02 |
Detector | LADI III |
Wavelength(s) | 3.0-3.9 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 57.952, 57.952, 100.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.400 |
R-factor | 0.2995 |
Rwork | 0.295 |
R-free | 0.34120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wut |
RMSD bond length | 0.029 |
RMSD bond angle | 2.085 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.530 | 2.060 |
High resolution limit [Å] | 2.000 | 2.400 | 2.000 |
Rmeas | 0.063 | 0.513 | |
Number of reflections | 12464 | 363 | 891 |
<I/σ(I)> | 25.2 | 2.8 | 3 |
Completeness [%] | 98.9 | 36.9 | 96.1 |
Redundancy | 9.7 | 2.2 | 3.2 |
CC(1/2) | 0.999 | 0.849 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 281 | 28% PEG 6000, 0.1 M citrate, pD 4.75 | |
1 | VAPOR DIFFUSION, HANGING DROP | 281 | 28% PEG 6000, 0.1 M citrate, pD 4.75 |