7O5Y

PilA minor pilin of Streptococcus sanguinis type IV pili

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9200
Spacegroup name	P 43 21 2
Unit cell lengths	93.320, 93.320, 181.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.920 - 1.770
Rwork	0.206
R-free	0.23800
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.839
Data reduction software	xia2
Data scaling software	xia2
Phasing software	CRANK2
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.320	1.800
High resolution limit [Å]	1.770	1.770
Number of reflections	78394	3841
<I/σ(I)>	12.1
Completeness [%]	99.8
Redundancy	12.5
CC(1/2)	0.995	0.508

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris pH 8; 0.1 M magnesium nitrate hexahydrate; 23 % (v/v) PEG 2000