7O5Y
PilA minor pilin of Streptococcus sanguinis type IV pili
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-22 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9200 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 93.320, 93.320, 181.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.920 - 1.770 |
Rwork | 0.206 |
R-free | 0.23800 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 0.839 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 93.320 | 1.800 |
High resolution limit [Å] | 1.770 | 1.770 |
Number of reflections | 78394 | 3841 |
<I/σ(I)> | 12.1 | |
Completeness [%] | 99.8 | |
Redundancy | 12.5 | |
CC(1/2) | 0.995 | 0.508 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris pH 8; 0.1 M magnesium nitrate hexahydrate; 23 % (v/v) PEG 2000 |