7O52

CD22 d6-d7 in complex with Fab m971

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033200
Spacegroup name	I 2 2 2
Unit cell lengths	61.478, 119.719, 241.672
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.823 - 2.410
Rwork	0.226
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7o4y
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (v1.14)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	7.531	2.550
High resolution limit [Å]	2.410	7.150	2.410
Rmerge	0.098	0.034	1.681
Rmeas	0.102	0.035	1.751
Number of reflections	35031	1469	5468
<I/σ(I)>	20.19	66.81	1.46
Completeness [%]	99.6	99.3	97.4
Redundancy	13.18	11.798	12.624
CC(1/2)	0.999	0.999	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2M ammonium sulphate, 0.1M HEPES pH 7.5