7O4X
Crystal structure of the PII-like protein PotN from Lentilactobacillus hilgardii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00001 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 80.151, 80.151, 79.484 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.080 - 1.650 |
| R-factor | 0.18022 |
| Rwork | 0.179 |
| R-free | 0.19945 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eg1 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.108 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.110 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.091 | 2.030 |
| Rmeas | 0.093 | |
| Number of reflections | 18721 | 2935 |
| <I/σ(I)> | 21.4 | 1.75 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 26.19 | 24.71 |
| CC(1/2) | 0.999 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.56 M Tri-Na Citrate pH 7 |
