7O4P

Cystal structure of Zymogen Granule Protein 16 (ZG16)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9731
Spacegroup name	P 32 2 1
Unit cell lengths	58.336, 58.336, 82.618
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.100 - 1.080
R-factor	0.1648
Rwork	0.165
R-free	0.17480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3apa
RMSD bond length	0.006
RMSD bond angle	0.977
Data reduction software	HKL-2000
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.101	1.119
High resolution limit [Å]	1.080	1.080
Rmerge	0.040
Number of reflections	69590	6920
<I/σ(I)>	11.6
Completeness [%]	99.0	99.58
Redundancy	3.1	3
CC(1/2)	0.999	0.411

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (HEPES), pH 7.0, 30 % v/v Jeffamine ED-2003