7O4M
Structure of Staphylococcus aureus m1A22-tRNA methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2017-09-24 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.225, 60.705, 63.128 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.760 - 1.300 |
| R-factor | 0.1163 |
| Rwork | 0.115 |
| R-free | 0.14760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kr9 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.768 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.800 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.040 | 0.254 |
| Rmeas | 0.045 | 0.285 |
| Rpim | 0.020 | 0.127 |
| Number of reflections | 56680 | 2836 |
| <I/σ(I)> | 17.5 | 1.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.8 | 4.8 |
| CC(1/2) | 0.990 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M ammonium acetate, 0.1 M Bis-Tris pH 5.5, 17% PEG 10,000 |
