7O4M

Structure of Staphylococcus aureus m1A22-tRNA methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	90
Detector technology	PIXEL
Collection date	2017-09-24
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	59.225, 60.705, 63.128
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.760 - 1.300
R-factor	0.1163
Rwork	0.115
R-free	0.14760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kr9
RMSD bond length	0.013
RMSD bond angle	1.768
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.800	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.040	0.254
Rmeas	0.045	0.285
Rpim	0.020	0.127
Number of reflections	56680	2836
<I/σ(I)>	17.5	1.9
Completeness [%]	99.9	99.9
Redundancy	4.8	4.8
CC(1/2)	0.990	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M ammonium acetate, 0.1 M Bis-Tris pH 5.5, 17% PEG 10,000