7O3K
Structure of a monomeric variant (L135E) of Sandercyanin fluorescent protein bound to biliverdin IX-alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-05 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.987 |
Spacegroup name | P 41 |
Unit cell lengths | 39.810, 39.810, 119.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.810 - 2.750 |
R-factor | 0.2324 |
Rwork | 0.231 |
R-free | 0.25050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ez2 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.7.7) |
Phasing software | PHENIX (1.19_4092) |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.810 | 2.900 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.109 | 0.553 |
Rmeas | 0.130 | 0.657 |
Rpim | 0.070 | 0.350 |
Number of reflections | 4795 | 703 |
<I/σ(I)> | 9 | 2.4 |
Completeness [%] | 99.2 | 98 |
Redundancy | 3.4 | 3.4 |
CC(1/2) | 0.993 | 0.804 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 26mg/mL 10% (w/v) PEG 1000, 10% (w/v) PEG 8000 |