7O2Y

Sandercyanin Fluorescent Protein variant V71E bound to biliverdin IX-alpha

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-05
Detector	MAR CCD 130 mm
Wavelength(s)	0.8729
Spacegroup name	P 41
Unit cell lengths	38.466, 38.466, 117.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.560 - 2.500
Rwork	0.173
R-free	0.25350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ez2
RMSD bond length	0.008
RMSD bond angle	1.613
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.840	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	41927	3399
<I/σ(I)>	10.4
Completeness [%]	99.6
Redundancy	6.8
CC(1/2)	0.990	0.306

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	11mg/mL 0.2M calcium chloride dihydrate, 20% (w/v) PEG 3350, pH 5.1