7O26
Complex-B bound [FeFe]-hydrogenase maturase HydE fromT. Maritima (5'dA + Methionine)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.880, 79.840, 86.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.810 - 1.500 |
R-factor | 0.1798 |
Rwork | 0.178 |
R-free | 0.21650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ciw |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.810 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 107892 | 8952 |
<I/σ(I)> | 5.28 | |
Completeness [%] | 99.5 | |
Redundancy | 6.9 | |
CC(1/2) | 0.988 | 0.453 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG; LiSO4; Tris pH 7.4 |