7O1S
Complex-B bound [FeFe]-hydrogenase maturase HydE fromT. Maritima (Wild-type protein)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-21 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.270, 79.700, 86.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.050 - 1.390 |
R-factor | 0.1562 |
Rwork | 0.155 |
R-free | 0.18040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ciw |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.050 | 1.480 |
High resolution limit [Å] | 1.390 | 1.390 |
Number of reflections | 102006 | 2180 |
<I/σ(I)> | 13.7 | |
Completeness [%] | 99.3 | |
Redundancy | 4.6 | |
CC(1/2) | 0.998 | 0.741 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG, LiSO4; Tris Ph 7.4 |