7O0S
Crystal structure of the N-terminal domain of CEP164(1-109) bound to camelid nanobody 36Z
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-04 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.97942 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 38.258, 50.558, 119.867 |
Unit cell angles | 90.00, 98.95, 90.00 |
Refinement procedure
Resolution | 59.200 - 1.700 |
R-factor | 0.2039 |
Rwork | 0.202 |
R-free | 0.24110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7o06 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.813 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0009) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.200 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.146 | 1.658 |
Rpim | 0.061 | 0.700 |
Number of reflections | 25042 | 1331 |
<I/σ(I)> | 6.1 | 1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.6 | 6.5 |
CC(1/2) | 0.995 | 0.798 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 292 | 0.18 M tri-ammonium citrate, 20 % (w/v) PEG 3350 |