7O0H
Structure of the foamy viral protease-reverse transcriptase dRH in complex with ds DNA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-14 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.033200 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.516, 107.558, 118.090 |
| Unit cell angles | 90.00, 98.72, 90.00 |
Refinement procedure
| Resolution | 48.840 - 3.090 |
| R-factor | 0.2326 |
| Rwork | 0.230 |
| R-free | 0.28530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MFV RT |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.280 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Number of reflections | 35709 | 5612 |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.994 | 0.564 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | PEG 3350, DL malic acid |
