7O0B
TRIM3 Filamin domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.017, 33.504, 58.844 |
| Unit cell angles | 90.00, 117.25, 90.00 |
Refinement procedure
| Resolution | 28.000 - 2.130 |
| R-factor | 0.2283 |
| Rwork | 0.227 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4umg |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.437 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.210 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.217 | 0.951 |
| Rmeas | 0.265 | 1.178 |
| Rpim | 0.149 | 0.687 |
| Number of reflections | 5379 | 428 |
| <I/σ(I)> | 3.82 | 1.15 |
| Completeness [%] | 96.7 | 77.72 |
| Redundancy | 3 | 2.7 |
| CC(1/2) | 0.966 | 0.482 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1M Na Cacodylate pH6.5, 1M Na citrate |
