7O05
Crystal structure of SARS-CoV-2 N-CTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.843, 44.818, 59.123 |
| Unit cell angles | 92.04, 96.20, 90.00 |
Refinement procedure
| Resolution | 44.790 - 1.940 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6wzo |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.828 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 58.740 | 44.790 | 2.040 |
| High resolution limit [Å] | 1.940 | 6.120 | 1.940 |
| Rmerge | 0.167 | 0.063 | 0.844 |
| Rmeas | 0.200 | 0.075 | 0.997 |
| Rpim | 0.109 | 0.040 | 0.527 |
| Total number of observations | 3317 | 16836 | |
| Number of reflections | 32080 | 1011 | 4753 |
| <I/σ(I)> | 6.2 | 13.5 | 2 |
| Completeness [%] | 96.2 | 95.5 | 96.6 |
| Redundancy | 3.4 | 3.3 | 3.5 |
| CC(1/2) | 0.984 | 0.992 | 0.704 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 0.1 M tri-Sodium citrate pH 4.5; 0.1 M bisTris pH 5.5; 25% PEG 3350 |
