7NZO
D-lyxose isomerasefrom the hyperthermophilic archaeon Thermofilum sp
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-02 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.770, 86.910, 54.030 |
| Unit cell angles | 90.00, 112.93, 90.00 |
Refinement procedure
| Resolution | 39.252 - 1.670 |
| Rwork | 0.179 |
| R-free | 0.21070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y0o |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.612 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.260 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Number of reflections | 43436 | 1266 |
| <I/σ(I)> | 19.4 | |
| Completeness [%] | 92.2 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.999 | 0.326 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 293 | 150 mM Potassium bromide, 30% w/v PEG 2000 MME |
