7NZI
TrmB complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | H 3 |
| Unit cell lengths | 177.480, 177.480, 42.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.180 - 3.100 |
| R-factor | 0.2336 |
| Rwork | 0.231 |
| R-free | 0.29090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fca |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.542 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.180 | 3.350 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.297 | 1.422 |
| Rmeas | 0.318 | 1.524 |
| Total number of observations | 70654 | |
| Number of reflections | 9049 | 1884 |
| <I/σ(I)> | 7.2 | 1.43 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.808 | 7.694 |
| CC(1/2) | 0.985 | 0.622 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 20% PEG4000, 20% Glycerol, 0.06M KAc, 0.08M (NH4)2SO4, 0.01M (CH3)3N |
