7NZ8

Crystal structure of mouse ADAT2/ADAT3 tRNA deamination complex 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-05
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00001
Spacegroup name	P 2 21 21
Unit cell lengths	52.291, 106.832, 130.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.440 - 2.120
R-factor	0.203
Rwork	0.201
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dh1
RMSD bond length	0.008
RMSD bond angle	0.880
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.120	2.120
Rmerge	0.180	2.420
Number of reflections	42256	6653
<I/σ(I)>	11.57	1.05
Completeness [%]	99.8
Redundancy	13.3
CC(1/2)	0.998	0.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% (W/V) PEG3350, 0.1 M Bis-Tris Propane pH 7.5, 0.2M KSCN