7NYN
Mutant Y526A of SH3 domain of JNK-interacting Protein 1 (JIP1)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96546 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 210.639, 62.485, 87.056 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.350 - 1.537 |
| Rwork | 0.138 |
| R-free | 0.17960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7nyk |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.864 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (7.1.007) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.350 | 1.630 |
| High resolution limit [Å] | 1.537 | 1.540 |
| Number of reflections | 329963 | 52785 |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 6.3 | |
| CC(1/2) | 0.990 | 0.310 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 0.1 M HEPES pH 7.5, 1-5 % PEG 400, 2-2.5 M ammonium sulfate |
