7NYM
Mutant V517A - SH3 domain of JNK-interacting Protein 1 (JIP1)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.8731 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.120, 84.254, 98.315 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.976 - 1.614 |
Rwork | 0.131 |
R-free | 0.19760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7nyk |
RMSD bond length | 0.011 |
RMSD bond angle | 1.680 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (7.0.078) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.976 | 1.518 |
High resolution limit [Å] | 1.450 | 1.454 |
Number of reflections | 45428 | 2272 |
<I/σ(I)> | 7 | 1.3 |
Completeness [%] | 94.0 | |
Redundancy | 12.5 | |
CC(1/2) | 0.990 | 0.460 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 0.1 M HEPES pH 7.5, 1-5 % PEG 400, 2-2.5 M ammonium sulfate |