7NYL
Mutant H493A of SH3 domain of JNK-interacting Protein 1 (JIP1)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.055, 45.551, 46.006 |
| Unit cell angles | 90.00, 104.27, 90.00 |
Refinement procedure
| Resolution | 44.586 - 1.950 |
| Rwork | 0.205 |
| R-free | 0.25450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7nyk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.673 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (7.0.078) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.550 | 2.030 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 8443 | 1083 |
| <I/σ(I)> | 6.9 | 1.18 |
| Completeness [%] | 96.3 | 77.9 |
| Redundancy | 8.4 | |
| CC(1/2) | 0.990 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 0.1 M HEPES pH 7.5, 1-5% PEG 400, 2-2.5 M Ammonium sulfate |
