7NYJ
Structure of OBP1 from Varroa destructor, form P3<2>21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-06 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98011 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 53.800, 53.800, 91.670 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.590 - 1.810 |
R-factor | 0.18832 |
Rwork | 0.186 |
R-free | 0.22609 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.829 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.600 | 1.950 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.080 | 1.070 |
Number of reflections | 13957 | 2889 |
<I/σ(I)> | 13.26 | 1.87 |
Completeness [%] | 100.0 | 100 |
Redundancy | 20 | 20 |
CC(1/2) | 0.999 | 0.742 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 2/3 of the initial condition (0.2M Cadmium Chloride, 2.2 ammonium sulfate) + 1/3 of PEG II screen (0.2M calcium chloride, HEPES 0.1M pH 7,5; PEG 4000 30% w/v). |