7NY7

Crystal structure of the Capsaspora owczarzaki macroH2A macrodomain in complex with ADP-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	BRUKER METALJET
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-14
Detector	Bruker PHOTON II
Wavelength(s)	1.34
Spacegroup name	P 32 2 1
Unit cell lengths	71.392, 71.392, 81.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.848 - 2.000
R-factor	0.163
Rwork	0.158
R-free	0.20540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iid
RMSD bond length	0.005
RMSD bond angle	0.948
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_3126: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.850	2.071
High resolution limit [Å]	2.000	2.000
Rmerge	0.040	0.810
Rmeas	0.051	0.840
Number of reflections	16593	1608
<I/σ(I)>	20.2	1.3
Completeness [%]	96.1
Redundancy	4.5
CC(1/2)	0.990	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2M ammonium tartarate dibasic 20% (w/v) PEG3350