7NWX

SARS-COV2 NSP5 in the presence of Zn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER IMUS MICROFOCUS
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-12
Detector	Bruker PHOTON III
Wavelength(s)	1.541
Spacegroup name	C 1 2 1
Unit cell lengths	114.620, 53.360, 44.650
Unit cell angles	90.00, 102.03, 90.00

Refinement procedure

Resolution	31.420 - 1.800
R-factor	0.2061
Rwork	0.204
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yb7
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.180	1.910
High resolution limit [Å]	1.800	1.800
Rmerge	0.120	0.950
Number of reflections	40960	1891
<I/σ(I)>	13.4	1.4
Completeness [%]	99.7
Redundancy	6.5
CC(1/2)	0.990	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium acetate, 20% PEG 3350, 1.5 mM zinc chloride