7NWN
A carbohydrate binding module family 9 (CBM9) from Caldicellulsiruptor kristjanssonii
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00003 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 172.671, 172.671, 172.671 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.191 - 1.970 |
Rwork | 0.180 |
R-free | 0.23010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i8u |
RMSD bond length | 0.013 |
RMSD bond angle | 1.811 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.191 | 2.110 |
High resolution limit [Å] | 1.970 | 1.970 |
Number of reflections | 31213 | 5694 |
<I/σ(I)> | 26.81 | |
Completeness [%] | 100.0 | |
Redundancy | 79.9 | |
CC(1/2) | 1.000 | 0.760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M phosphate-citrate and 40% polyethylene glycol 300 |