7NVQ

Aerosol-soaked human cdk2 crystals with Staurosporine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-24
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	53.889, 69.420, 74.419
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.760 - 2.050
R-factor	0.2402
Rwork	0.237
R-free	0.28790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4erw
RMSD bond length	0.006
RMSD bond angle	1.419
Data reduction software	MOSFLM
Data scaling software	SCALA (CCP4_3.3.22)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	74.420	74.420	2.160
High resolution limit [Å]	2.050	6.480	2.050
Rmerge	0.129
Rmeas	0.140	0.040	1.001
Number of reflections	15604	629	1902
<I/σ(I)>	6.8
Completeness [%]	87.1	95.89	75.13
Redundancy	6.4
CC(1/2)	0.993	0.999	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	10% PEG3350, 0.1M MES pH 7.0