7NUG

Influenza A/California/07/2009(H1N1) endonuclease in complex with orientin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-25
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.54187
Spacegroup name	P 64 2 2
Unit cell lengths	74.361, 74.361, 126.462
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.150 - 1.900
R-factor	0.1978
Rwork	0.195
R-free	0.25050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ya5
RMSD bond length	0.009
RMSD bond angle	1.531
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.150	42.150	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.089	0.033	1.192
Rmeas	0.094	0.034	1.309
Total number of observations	157145
Number of reflections	16879	240	1211
<I/σ(I)>	15.29	60.59	1.6
Completeness [%]	99.5	96	99.3
Redundancy	9.31	15.033	6.192
CC(1/2)	0.998	1.000	0.582

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	MDP, PEG 1000, PEG 3350, Sodium HEPES, MOPS (acid), Magnesium chloride, Calcium chloride