7NUE

Crystal structure of mouse PRMT6 in complex with inhibitor EML736

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-13
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	41.820, 118.531, 71.957
Unit cell angles	90.00, 103.06, 90.00

Refinement procedure

Resolution	38.530 - 2.000
R-factor	0.198
Rwork	0.196
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c03
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.260	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.097	0.629
Rmeas	0.112	0.737
Rpim	0.055	0.376
Number of reflections	45652	3074
<I/σ(I)>	14.4	2
Completeness [%]	98.7	91.4
Redundancy	3.8	3.3
CC(1/2)	0.996	0.679

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	PEG 3,350 20%, NaCHOO 200 mM, Tris-HCl pH 7.5 100 mM